Peter Attia· MD
the crisper cast 9 system has I think of it as an Achilles heel of a doubl stranded DNA break so it fundamentally in terms of being able to make the correction has to cut the DNA cutting the two strands of the DNA to make the correction and that fundamentally leads to some instability as the cell repairs that process which you use the word Fidelity which I like the use of that term because it really is all about Fidelity and potential off-target effects where you inadvertently introduce other than the intended correction other genetic changes and sometimes those other genetic changes can cause Mischief we call them of Target effects so things where they may inadvertently destroy the gene cause other changes to the gene cause other changes to other genes that you weren't even trying to Target but it can lead to problems of essentially promiscuity or inaccuracy or low Fidelity this is to me in my opinion the Achilles heal in terms of that particular system