Optimal dose, duration, and timing for senolytics have not been determined by original researchers.

“I asked vanerson had he measured the number of senescent cells in his treated mice no we're planning to do that but what was the phenotypic change in the mice when you did this experiment oh when I I could I didn't want to do an expensive Lipan experiment with with an alleged anti anti-colitic drug until I knew that it was depleting sessen so how long did you treat for I used their protocol and we I asked them I asked Darren Baker how what is the dose how long do you treat the mice and how long after you add the the drug should you wait before you detect the removal of senescent cells and his answer astonishingly was we don't know we've never looked at that”