Peter Attia· MD
so so um there's different Technologies to do that for self-re DNA um the way it's done is um and this adds complexity to to the to the chemistry is you pre-treat the DNA in a way that encodes the methylation status in the atg sequence and then you just use a sequencer that can only see atcg but because you've encoded the information you can then deconvolute it and infer which sites were methylated um uh to just to be a little more specific um there's chemicals that will for example um deaminate uh a a cytosine that's not methylated and then that deaminated cytosine um effectively turns into a osil which is a fifth letter in RNA and then when you copy the DNA and you amplify it prior to the sequencing it amplifies as a t because a u um when it's copied by um our DNA plase becomes a t and then you end up with a sequence where you expect to see C's and you see a t and if you see a t there then you know that aha came from a and the U is an unmethylated C brilliant yep right and if the c um was not changed then you say then that must have been a site that was methylated because you'll see G's opposite them right well oh sorry if if the C was methylated you'll see the G opposite because you won't turn it to the urisol right right BR so um uh yeah so that that technique is called bisulfide sequencing