Depletion of neurotransmitters at the neuromuscular junction can lead to profound weakness and fatigue, requiring time to reconstruct and replenish them.

“closer to neuronto neuron junctions or the neuromuscular junction between the neuron and the muscle itself you have vesicles of neurotransmitter you pump enough of those in you get the cool stuff of communication you can run low on neurotransmitter and then like the electrical signal arrives and the neurotransmitter is like "Sorry not enough of us to do anything." So you experience profound weakness which is expressed as uh or fatigue expressed as weakness and you need time to reconstruct a lot of those neurotransmitters place them into vesicles have those vesicles transllocate to the synaptic cleft and then like sit there and get ready and that is a process that typically happens rapidly but if you really exhaust it it can happen over some time”