Fisetin was tested as a potential senolytic drug to see if removing senescent cells would extend lifespan.

“it turns out it didn't remove any ccent cells either um what we can do is you can there are quite a number of surface markers like p16 that the sessen cell gurus say this will tell you whether you've got sin essent cells they do go up with age they go up with age and we looked at three different tissues ises that is um Paul Robins lab and Jim Kirkland's lab we we sent them tissue it was blind so they wouldn't know which ones were controls and which ones were treated they sent us back this number of beta galac aidas positive cells this number of P6 positive cells this number this amount of p21 and when we unblinded it there was no effect of fistin on brain on liver on muscle on kidney um at either lab for any marker that we looked at”

“however it turns out it didn't remove any Cent cells either what we can do is you can there are quite a number of surface markers like p16 that the ccent cell gurus say this will tell you whether you've got ccent cells they do go up with age they go up with age and we looked at three different tissues that is Paul Robins lab and Jim Kirkland's lab we we sent them tissue it was blind so they wouldn't know which ones were controls and which ones were treated they sent us back this number of beta galactosidase positive cells this number of p16 positive cells this number this amount of p21 and when we unblinded it there was no effect of fistin on brain on liver on muscle on kidney at either lab for any marker that we looked at”

“so fan was given to us as a drug to test the hypothesis that if you remove senent cells from mice by giving them ftin they would live longer a very I thought it wouldn't work but it was a very reasonable and important thing to do”