Platelet-rich plasma (PRP) preparation involves activating platelets with calcium and concentrating them.

“you can usually use some form of calcium like calcium gluconate or calcium chloride and so you just and you dilute the platelets obviously when you're in jibt before you inject them or do you inject them in that constant so I go from that a DCC of initial blood draw and I end up with about eight CC's total of PRP and that's fully concentrated platelet there's a little bit of platelet poor plasma in there but I've gotten rid of most of that platelet poor plasma so it's platelets suspended in plasma but it's highly concentrated and many fold more than what you'd have in your serum”