Cancer cells grown in a dish are subjected to different environmental selection forces compared to in vivo, making extrapolation of molecular biology study results to in vivo conditions potentially unreliable.

“take cancer cells out of a patient put them in a dish and now you start to grow from culture will know that they're no longer uh being attacked by the immune system they no longer have to worry about androgenesis they i mean it's an entire world they don't have the edge effects that you described earlier where some of them have a selective advantage vis-a-vis access to substrate whereas others are more competing with the tumors at the inside sure so they they may be they're subjected to entirely different environmental selection forces now we say we take those tumors out we do molecular biology studies and we say oh well this came from a lung cancer that means lung cancer must interact with this to do this when in fact we're dealing with cells that have evolved far past you know they're they're far different from the cancer cells that were present”