Studying glycogen depletion via muscle biopsies is impractical due to the heterogeneity of glycogen stores.

“even in muscle biopsies you will see that if you basically do sampling just across one muscle so even yeah it's so heterogenic that even in well trained a so if you wanted to do it you would need to biopsy multiple times multiple sites simultaneously or even make somebody radioactive and start to do nuclear magnetic re res yeah measurements on on people yeah so so it's it's something it's so impractical to really study”