Enzymes upregulated by sulforaphane have a half-life on the order of days to weeks, remaining active for a significant period after ingestion.

“What you are doing when you take sulforaphane is you're up-regulating a bunch of enzymes. And those enzymes have a rather long, comparatively, a rather long half-life meaning they don't lose their punch. They don't get deactivated or get broken down all that fast. And their half-lives are on the order of days, maybe even weeks for some of them, but certainly a few days is a reasonable guess for many of them. So once you boost all these enzymes, they stay up-regulated, in other words, more active for a period of time and then it goes away.”

“the fact that the 12 weeks there was no exhausting of the the the up regulation of these important detoxification enzymes — that's really profound that that they were maintained within that whole 12 week it wasn't like it was a short-term effect that went away”

“yeah so i do i mean these these obviously there's some time lag between the time you ingest the glucoraphanin or sulforaphane and the time the enzymes are upregulated or respond it's quite short though”

“so you know we know that a day after taking sulforaphane we see these enzymes elevated and um and and also i can't remember uh two or three days has been a time point that that we've looked at specifically but um but others have i think in the clinic”

“one thing we do know from our work in china with with a study of air pollution and the effects of sulforaphane on that is that you don't fatigue and this is fortunate but you don't fatigue this the biochemical response system so we gave for 12 weeks we gave daily glucoraphanin and sulforaphane and monitored the upregulation of the this protective response — and did not really see any fatiguing of the of the the response of that system”