Heat can deactivate the epithiospecifier protein, which directs glucoraphanin towards non-anti-carcinogenic sulforaphane nitriles.

“however glucoraphanin not only forms sulforaphane it can also form sulforaphane nitriles which do not contain the anti-carcinogenic properties that sulforaphane has a key determinant of whether the myrosinase enzyme will form sulforaphane versus sulforaphane nitriles is the epithiospecifer protein which is a non-catalytic cofactor of myrosinase the epidio specifer protein shifts the pathway towards sulforaphane nitriles”