Gut bacteria's ability to convert glucoraphanin to sulforaphane can be regained within weeks after depletion by enemas and antibiotics.

“So in fact, we did show that, this was a number of years ago now, we showed that if volunteers took a Fleet enema, self-administered enema, which reduces the number of bacteria, certainly in their large intestine, by, I don't know, five or six orders of magnitude, and took a preoperative antibiotic course, in other words, something that one would take prior to having intestinal surgery, they essentially wiped out most of the bacteria in their intestines.”

“absolutely all of us do and i mean that all however many hundred of you there are in this call all have myrosinase containing bacteria in your gut unless you've just blasted yourself with antibiotics and had a you”

“so i think that's so interesting that we have the enzyme in our gut meaning are meant to get the precursor to convert it into sulforaphane we contain the enzyme in our gut i i just love that”