Increased PGC-1 expression in muscle was observed in mice on a standard diet with TRE, suggesting a potential increase in mitochondrial function.

“But surprisingly in the same mice we saw there is less glycogen in muscle. And that's kind of counterintuitive. You might think that, "Well, the muscles may be loaded with glycogen, so that's why their lean mass went up." But at the same time what we think is maybe, because they have less glycogen, we know that if the muscle has less glycogen than after normal activity, then glycogen may be completely depleted. And when they eat, they have a much better feeding response. So that means a much better glycogen synthesis response.”