Animal studies show that depleting endocannabinoid levels can cause anxiety, which can be reversed by boosting endocannabinoids or administering THC.

“they did demonstrate that so you could deplete endoc canabo levels and you got the emergence of an anxiety state so then you could give drugs that would boost the endoc canabo to normalize this um so again it kind of fit with the idea but then they did one key study where then they gave THC and saw could THC fell in the Gap and they found that like boosting endoc canabo giving THC on a background of low endoc canabo was able to reverse that anxiety phenotype and bring it back into more of the normal range”