Bryan Johnson's biomarker assessment includes cognitive function metrics such as brain age score, functional connectivity strength, global connectivity index, hemodynamic response amplitude, hemodynamic response latency, resting-state oxygenated hemoglobin concentration, resting-state deoxygenated hemoglobin concentration, total hemoglobin concentration, oxygen saturation, cerebral blood volume, cerebral blood flow, neurovascular coupling index, prefrontal cortex oxygenation, parietal cortex oxygenation, temporal cortex oxygenation, occipital cortex oxygenation, task-evoked hemodynamic response, temporal autocorrelation of hemodynamics, functional brain network modularity, inter-regional coherence, brain lateralization index, oscillatory power, phase synchronization index, mean reaction time, reaction time variability, heart-rate variability, and resting heart rate.

“Cognitive + Brain age score + Functional connectivity strength + Global connectivity index + Hemodynamic response amplitude + Hemodynamic response latency + Resting-state oxygenated hemoglobin (HbO) concentration + Resting-state deoxygenated hemoglobin (HbR) concentration + Total hemoglobin (HbT) concentration + Oxygen saturation (O₂Sat) + Cerebral blood volume (CBV) + Cerebral blood flow (CBF) + Neurovascular coupling index + Prefrontal cortex oxygenation + Parietal cortex oxygenation + Temporal cortex oxygenation + Occipital cortex oxygenation + Task-evoked hemodynamic response (HbO/HbR) + Temporal autocorrelation of hemodynamics + Functional brain network modularity + Inter-regional coherence + Brain lateralization index + Oscillatory power (slow cortical fluctuations) + Phase synchronization index + Mean reaction time (during cognitive tasks) + Reaction time variability + Heart-rate variability (HRV) + Resting heart rate”