MCF-7 breast cancer cells incubated in glucose alone produce high levels of lactate within six hours, consistent with the Warburg effect.

“So when you add glucose by itself, we looked into the media and it was a very high lactate levels. Looks like on your graph, it's almost 30 millimole. Yes. It was almost 30 millimole in the six hours. And because lactate is also used by the cells for energy purposes, over time we expect it also to see, but we still see about 25 millimolar. This is the water borer effect. This is what water borer observed, incubating in cells and say, They use a lot of glucose, but why in the world is this lactate? It's amazing it's that high.”