Mice subjected to severe caloric restriction for three days prior to a procedure involving clamping blood supply to the legs survived, while ad-lib fed mice died.

“the third piece would that was interesting was the group that were just starved transiently actually had a faster recovery”

“look at j mitchell's stuff where they do a one day fast prior to a femoral artery ligation and a reperfusion where the mouse that was just fed normally through the insult they all die the mice that had 24 hours of fasting prior to a lethal reperfusion injury either all or mostly live”

“they took another group that were ad-lib fed but i think three days prior to the surgery were severely calorically so so each of them then had the same procedure which was a laparotomy with a ligation of the femoral arteries for a period of time and then a reperfusion”

“it actually cut off blood supply of the kidneys and he also had another one that they did the liver same result though and you're exactly right they did these ones that have been calorically restricted their whole lives the ones that have been that were ad-libbed and then the ones that have been fasted for they did some that have been fast uh restricted for a few weeks and then some that have been just fasted water only fast for a day two or three days”

“so so each of them then had the same procedure which was a laparotomy with a ligation of the femoral arteries for a period of time and then a reperfusion so what you know for the folks listening what that means is you clamp off all the blood supply to the lower part of the leg and then you know basically just before the animal is about to die you let it you let the blood flow again but because of all of the ischemic damage to the tissue all the tissue damage due to no oxygen you create such an injury to the animal that I believe all of the ad-lib animals died from that but yet the two groups that were calorically restricted won its entire life and one just for three days survived suggesting that just that period of caloric restriction could produce a similar benefit”

“what he did the it actually cut off blood supply of the kidneys yeah another one that they did the liver okay same result though and you're exactly right they did these ones that have been calorically restricted their whole lives”