Peter Attia· MD
The observation was made in 1987. I bumped into Peter Downs, who's one of the best lipid chemists in the world, and had a meeting at Cold Spring Harbor, and I told him this result, and I said, I know you must have standards for inositol degradation, so can we work together and So we knew it had to be some site other than the four position, but it could have been the five. It could have been the three. Yeah. Was there a chance it could have been, you were looking at four versus four plus five, four versus four plus five? No, because we knew at four plus five, it would migrate about three inches different. So that would look totally different. Yeah. So the null hypothesis is you're just looking at four and your assay is a little dirty, but you're thinking, no, it's something else. Of course, we repeated it multiple times, and we also did HPLC separation, reverse phase, every way you could look at it. And in every way we did it, they were chemically different. They migrated differently and numerous. The nature paper, we only put that one slide, that one figure of the slightly different migration. Because from there on, we did the chemistry to prove that it was a three position.