2-Arachidonoylglycerol (2-AG) has lower affinity for cannabinoid receptors than anandamide but elicits a more robust intracellular signaling response upon binding.

“the way anandamide binds the receptor is it's what we would call a high Affinity but low efficacy um Agonist or molecule at least and what I mean by that is very low levels of anandamide are required to actually bind to the receptor but once it binds its ability to stimulate a biological response in that neuron is kind of caps out pretty fast so it doesn't have like a sledgehammer effect”