Chronic cannabis users tend to have fewer CB1 receptors in the brain, although the functional outcome of this downregulation is unclear.

“chronic cannabis users do have some down sorry I have to interrupt pet positron emission tomography not pets — um don't although people get their pets high and we don't know what those um pets think about that not good don't get dogs if also High one can assume a lot of things about what your pet is thinking while also High um sort of half joke there um but yes positron emission tomography is one way to assess their binding of um drugs within the brain as well as activity of endogenous um neurotransmitters neuromodulators such as um anandamide um dopamine Etc yeah so so a typical pet study in a human looking at this they'd give a molecule that's radi labeled that will bind a CB1 receptor you can scan them um and then look at the emission rates of the radiation to get an idea of the density of receptors that are in the brain chronic cannabis users tend to have less”