In OB mice, connecting circulation to DB mice caused dramatic weight loss, cessation of eating, and normalization of body fat within weeks.

“Coleman was able to show that so here you had animals that looked a great deal like each other at least in terms of the overeating and the low energy expenditure one was prone to get diabetes and the other not and what Coleman ultimately did was to join the circulation of the ob mouse to a wild-type animal and the DB or diabetes mouse to a wild-type animal user like a live para bioessence it's a lot that's the technical term for it as a para bios's and he showed that if you hooked an OB mouse up to a wild-type mouse in this way that the OB Mouse would correct its hyperphagia its excess food intake and actually begin to lose weight”

“did they ever do para bios's between the OB and the DB yes that was done as well okay because that would that should fix the OB it did but not the DB correct okay which is another great and elegant example that one of them had the ligand deficiency the other had the receptor deficiency”

“if you hooked an OB mouse up to a wild-type mouse in this way that the OB Mouse would correct its hyperphagia its excess food intake and actually begin to lose weight so something presumably in the wild-type Mouse some humeral factor some hormone some something must have been missing in the OB OB Mouse that when given to it corrected its phenotype correct”

“what happened when you did the para bios's with the DB Mouse the DB Mouse you got the opposite situation where the wild-type animal began to lose weight and actually stopped eating and would die of starvation basically whereas the DB Mouse just went merrily on its way eating and remaining obese”