Consuming more than approximately 0.25 grams of protein per pound in a single sitting may lead to protein oxidation rather than utilization for muscle protein synthesis.

“and really the totality of evidence would suggest it's probably around 55 grams so regardless of whether or not somebody does resistance training you can ingest about 50 gram of protein again four calories per gram um typically um into muscle protein synthesis the rest is oxidized”

“To maximize muscle protein synthesis, we really haven't seen doses beyond 50- ish grams, 30 or so, to 50.”

“My colleague, Brad Schoenfeld, and I, we scoured the literature, and we wrote this paper on 'What is the maximal anabolic dose of protein per meal for the goal of muscle building?' And we boiled it down to somewhere between 0.4 to roughly 0.6 grams per kilogram of body weight. And so, in freedom units, we're talking 0.2 to 0.25 grams per pound, and that is what appears to max out muscle protein synthesis.”

“if you look at direct amino acid oxidation so I I did this I I pulled up a bar of direct amino acid oxidation which basically is like if you feed a certain amount of protein if you're feeding increasing levels of protein and you look at the oxidation levels of a specific amino acid it'll be kind of flat and then you'll hit an inflection point and it'll kind of go up linearly and they've said okay that inflection point right there where you start increasing amino acid oxidation that's obviously wasteful right that that that's extra protein you don't need anym than where that inflection point is and then if you look at what actually increases muscle protein synthesis it's right at where amino acids start getting oxidized”

“if you consume too much protein in one sitting and it's typically more than about 0.25 grams per pound is the general thinking you're going to end up oxidizing some of that protein so it's not that it's harmful it's just that you're not getting the amino acids you need for muscle protein synthesis”

“that inflection point right there where you start increasing amino acid oxidation that's obviously wasteful”

“if you consume too much protein in one sitting uh and it's typically more than about 0.25 grams per pound is the general thinking you're going to end up oxidizing some of that protein so it's not that it's harmful it's just that you're not getting the amino acids you need for muscle protein synthesis”

“In humans, 20–25 grams of protein maximally stimulates muscle protein synthesis.”

“about 0.25 grams of protein per kilogram body weight in a single meal provides a saturating dose of Lucine and amino acids to stimulate muscle protein synthesis for up to 6 hours”