David Sinclair· PhD
When those maintenance systems were blocked (Sirt3/PINK1/Parkin), NMN no longer worked Cells were treated with 3-TYP, a selective inhibitor of Sirt3. The presence of 3-TYP abolished the facilitatory effect of NMN on autophagic flux, resulting in the stress response (ie. re-accumulation of autophagosomes and a significant reduction in autolysosome formation)